- Mobile Phase
- Stationary Phase
- Solvent Purity
- Layer thickness and binders in the layers.
- Size and shape of developing chamber.
- Amount of sample to be spotted.
- Chamber saturation.
- Size of spot applied.
- Solvent level and development level in the chamber.
- Relative humidity and temperature.
- Development distance.
- Mode of development.
Tuesday, 11 December 2012
Factors affecting TLC or HPLC separation
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