Tuesday, 11 December 2012

Factors affecting TLC or HPLC separation

SOME OF THE FACTORS WHICH AFFECTS TLC OR HPLC ARE AS FOLLOWS:-

  1. Mobile Phase
  2. Stationary Phase
  3. Solvent Purity
  4. Layer thickness and binders in the layers.
  5. Size and shape of developing chamber.
  6. Amount of sample to be spotted.
  7. Chamber saturation.
  8. Size of spot applied.
  9. Solvent level and development level in the chamber.
  10. Relative humidity and temperature.
  11. Development distance.
  12. Mode of development.
These are some of the factors affecting TLC or HPLC seperation.

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