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What are the Applications of Thin Layer Chromatography - TLC ?

 Some of the applications of thin layer chromatography - TLC are discussed below. So let us check out the applications to learn more about TLC. Some of the Applications of TLC are: Thin-layer chromatography is ideal for quick screening of plant extracts, dyes, and pharmaceutical compounds. TLC can separate pigments and chlorophylls in plant extracts for botanical studies. It identifies synthetic and natural dyes in textiles and artworks. It is also used to detect counterfeit drugs by comparing standard and sample compound profiles. TLC is used in teaching labs to demonstrate compound separation visually. It monitors the reaction progress in organic synthesis through spot comparison. In herbal medicines it also analyzes alkaloids, essential oils, and phenolic compounds. TLC compares purity of pharmaceutical samples using reference standards. It is generally employed in food testing to identify colorants and sweetener adulterants. It also serves as a low-cost method for preliminary c...

Advantages and Disadvantages of Thin Layer Chromatography TLC

 Some advantages and disadvantages of thin layer chromatography are discussed below. So let us check out its advantages and disadvantages to know more about thin layer chromatography. Some Advantages of Thin Layer Chromatography TLC are: Thin Layer chromatography is a rapid analysis method for complex mixtures. Minimal sample preparation. It has high separation efficiency. Low solvent consumption in thin layer chromatography. It requires small sample volumes. Cost effective method. Instrumentation is simple. Useful for wide range of applications. It is compatible with various detection methods. TLC is easy to perform and interpret.  Some Disadvantages of Thin Layer Chromatography TLC are: Thin layer chromatography has limited sample capacity. It has low resolution compared to other methods. It has limited reproducibility. TLC has limited sensitivity for some compounds.  Trained person is required to operate. High cost of stationary phase. Limited selectivity for certain a...

TLC Application | Some Applications of Thin Layer Chromatography

Advantages of Thin Layer Chromatography TLC

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Steps Involved in HPTLC

Factors affecting TLC or HPLC separation