How does thin layer chromatography separate chemical compounds?
Some points on thin layer chromatography to separate cheemical compounds:
- Thin layer chromatography separates compounds based on their different rates of movement over an adsorbent surface under a solvent.
- As the solvent rises through the stationary layer by capillary action, each compound is carried along at a speed determined by its attraction to the surface and its solubility in the solvent.
- Substances that bind more strongly to the adsorbent move slowly, while those with weaker attraction travel farther with the solvent front.
- This difference in travel distance creates physical separation of the mixture into individual components along the length of the plate.
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