Some advantages and disadvantages of high performance liquid chromatography are discussed below. So let us check it its advantages and disadvantages to know more about HPLC.
Some advantages of HPLC chromatography:
- HPLC has high resolution and speed of analysis.
- High surface area.
- It has high pressure gradient.
- It has wide range of stationary phases.
- Precise flow rate control.
- Senstive detection methods.
- Low sample method requirement.
- Accurate peak identification using HPLC.
Some disadvantages of HPLC chromatography
- HPLC has high cost.
- High quality components are needed.
- The solvents and columns used in HPLC are expensive.
- Regular maintenance and calibration is needed which add extra cost.
- Sophisticated software is required for data analysis.
- Research and development cost.
