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Importance of Thin Layer Chromatography (TLC)
Some points on importance of thin layer chromatography are:
- Thin layer chromatography is important because it provides a simple and effective method for separating and identifying chemical compounds.
- It separates the components of a mixture based on their different interactions with the stationary phase and the solvent, allowing each substance to move a unique distance on the plate.
- The resulting pattern of distinct spots makes it possible to compare unknown compounds with known standards and determine their identity using Rf values.
- Because the technique requires only small sample quantities and gives results quickly, it is widely used for purity testing, reaction monitoring, and preliminary analysis in chemical and pharmaceutical laboratories.