Thursday 13 December 2012

TLC Application | Some Applications of Thin Layer Chromatography

Various applications of Thin Layer Chromatography (TLC) are as follows:

  1. Purity of  any sample : Purity of sample can be carried out with TLC. Direct comparison  is done between the sample and the standard or authentic sample; if any impurity is detected, then it shows extra spots and this can be detected easily.
  2. Identification of compounds: Thin layer chromatography can be employed in purification, isolation and identification  of natural products like volatile oil or essential oil, fixed oil, waxes, terpenes, alkaloids, glycosides, steriods etc.
  3. Examination of reactions: Reaction mixture can be examined by Thin layer chromatography to access whether the reaction is complete or not. This method is also used in checking other separational processes and purification processes like distillation, molecular distillation etc.
  4. Biochemical analysis: TLC is extremely useful in isolation or separation of biochemical metabolites or constituent from its body fluids, blood plasma, serum, urine etc.
  5. In chemistry: TLC methodology is increasingly used in chemistry for the separation and identification of compounds which are closely related to each other. It is also used for identification of cations and anions in inorganic chemistry.
  6. In pharmaceutical industry: Various pharmacopoeias have adopted TLC technique for detection of impurity in a pharmacopoeial  chemical. 
  7. Various medicines like hypnotics, sedatives, anticonvulsant tranquillisers, antihistaminics, analgesics, local anaesthetics, steroida have been tested qualitatively by TLC method.
  8. One of the most important application of TLC is in separation of multicomponent pharmaceutical formulations.
  9. In food and cosmetic industry , TLC method is used for separation and identification of colours, preservatives, sweetening agent, and various cosmetic products.
This are some of the applications of Thin layer Chromatography (TLC)

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