Wednesday 12 December 2012

Advantages of Thin Layer Chromatography TLC

Advantages of Thin layer chromatography
Advantages of Thin layer Chromatography (TLC)

Some of the advantages of Thin Layer Chromatography (TLC) are as follows:-

  1. Less equipment is required.
  2. Very little time for separation is required.
  3. It is more sensitive.
  4. The lower detection limit of analytical sample in TLC is approximately one decimal lower than that is paper chromatography and very small quantities of sample is sufficient for analysis.
  5. Spraying with corrosive agents for identification is also permitted which is not possible in paper chromatography as cellulose gets destroyed.
  6. This method is used for adsorption, partition, ion exchange chromatography as there is wide range of adsorbents available.
  7. The individual samples do not get diffused as compared to paper chromatography hence sensitivity of detection is more.
  8. The components which are separated can be recovered easily by scratching the powdery coating of plate and quantitative separation of spots or zone are possible.
  9. It is possible to visualize the components for identification by UV light as the inorganic adsorbent background does not floresce.
  10. This method can be applied to preparative separation with the help of thicker layers of adsorbents.

Thank You


Popular Posts