How does thin layer chromatography help in identifying compounds?
Some points on thin layer chromatography in identifying compounds:
- Thin layer chromatography identifies compounds by comparing the position and pattern of sample spots with known reference standards.
- After development, the sample and standard are visualized under suitable conditions such as ultraviolet light or chemical staining to make their spots clearly visible.
- When the sample spot appears at the same relative position and shows a similar appearance as the reference spot, the compound is considered to be the same substance.
- This comparison method allows unknown components in a mixture to be recognized without requiring complex instrumentation or advanced analytical techniques.
Find out more points on importance of thin layer chromatography to learn more about it.