How does thin layer chromatography help in identifying compounds?

Some points on thin layer chromatography in identifying compounds:

  • Thin layer chromatography identifies compounds by comparing the position and pattern of sample spots with known reference standards. 
  • After development, the sample and standard are visualized under suitable conditions such as ultraviolet light or chemical staining to make their spots clearly visible. 
  • When the sample spot appears at the same relative position and shows a similar appearance as the reference spot, the compound is considered to be the same substance. 
  • This comparison method allows unknown components in a mixture to be recognized without requiring complex instrumentation or advanced analytical techniques.

Find out more points on importance of thin layer chromatography to learn more about it.