Saturday 11 March 2023

Advantages and Disadvantages of Thin Layer Chromatography TLC

 Some advantages and disadvantages of thin layer chromatography are discussed below. So let us check out its advantages and disadvantages to know more about thin layer chromatography.

Some Advantages of Thin Layer Chromatography TLC are:

  1. Thin Layer chromatography is a rapid analysis method for complex mixtures.
  2. Minimal sample preparation.
  3. It has high separation efficiency.
  4. Low solvent consumption in thin layer chromatography.
  5. It requires small sample volumes.
  6. Cost effective method.
  7. Instrumentation is simple.
  8. Useful for wide range of applications.
  9. It is compatible with various detection methods.
  10. TLC is easy to perform and interpret. 

Some Disadvantages of Thin Layer Chromatography TLC are:

  1. Thin layer chromatography has limited sample capacity.
  2. It has low resolution compared to other methods.
  3. It has limited reproducibility.
  4. TLC has limited sensitivity for some compounds. 
  5. Trained person is required to operate.
  6. High cost of stationary phase.
  7. Limited selectivity for certain analytes.
  8. TLC may require multiple development solvents. 

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