Friday, 5 October 2012

DETECTOR FOR HPLC


About Detector For HPLC:-

There are many Detectors used for HPLC. Let us see what are the different Detectors for HPLC and Some of the information related to detectors. List of different detectors are as follows


  • Ultra Violet Detector --(190-400nm)
  • Photo Diode Array (PDA)-- (Entire range products)
  • Refractive Index Detector--(For Compounds not contains Chromophores)
  • Fluorescence Detector --(Compounds that Fluoresce)
  • Mass Spectrometry
  • Evaporative Light Scattering Detector (ELSD)
  • Multi Angle Light Scattering Detector
  • Conductivity Detector
  • Optical Rotation Detector
  • Electro Chemical Detector
  • Chemiluminescence Detector
  • Nuclear magnetic Resonance Detector
  • Near Infrared Detector--(700-1100nm)


UV Is Widely used Detector among all the Detectors. But Aternative Detector used when:
  1. Little or no UV Absorbance
  2. Analyte concentration is too less for UV Detection
  3. Sample Interferences
  4. Qualitative structural information is required.
Let us check some of the features that HPLC Detectors must have:
  1. It should give response  to solute concentration (linear response)
  2. It should never response to mobile phase.
  3. It should not be affected where there is sometimes variation in flow rate as well as temperature.
  4. It should response to all compounds in a mixture.
Detectors affect the response in three ways:
  1. Sensitivity
  2. Selectivity
  3. Base line noise



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