What are the Applications of Affinity Chromatography?

 Some of the applications of affinity chromatography are given below. So let us find out the applications point wise to learn more about affinity chromatography.

Some of the applications of affinity chromatography are:

1. Affinity chromatography is sued to isolate specific proteins or enzymes using selective binding.
2. This chromatography purifies antibodies by binding them to antigen-linked resins. 
3. It isolates enzymes using immobilized substrate or inhibitor molecules. 
4. It separates recombinant proteins with affinity tags like His-tag or GST. 
5. It also concentrates low-abundance proteins from complex biological samples. 
6. It is used in immunology labs for purification of immunoglobulins and cytokines.
7.  It removes host cell proteins during biopharmaceutical purification. 
8. Affinity matrices are designed to capture specific ligands with high selectivity. 
9. It helps validate protein interactions in structural and functional biology studies. 
10. Affinity chromatography also supports vaccine production by isolating antigens from culture media. 
11. It is essential in proteomics workflows for target-specific sample enrichment.