What are the Applications of Size Exclusion Chromatography?

 Some of the applications of size exclusion chromatography are listed below. So let us check list of applications to know more about size exclusion chromatography. 

Some of the applications of size exclusion chromatography are:

1. Size exclusion chromatography is generally use to separate molecules by size for protein and polymer analysis.
2. It separates proteins, peptides, and macromolecules based on molecular weight. 
3. It is used to analyze polymer distribution and average molecular mass. 
4. It helps determine protein aggregation in stability and formulation studies. 
5. SEC distinguishes between monomers, dimers, and oligomers in biopharmaceutical samples. 
6. It is used in gel filtration experiments for buffer exchange and desalting. 
7. It also evaluates molecular size distribution in synthetic and natural polymers. 
8. It purifies DNA fragments or virus particles without altering structure or function. 
9. SEC supports quality control by detecting size-related degradation products. 
10. It allows estimation of unknown molecular weights using calibration standards. 
11. Size exclusion chromatography is non-destructive and preserves biological activity of eluted components.