What are the Applications of Gel Filtration Chromatography?

Some of the applications of gel filtration chromatography are discussed below. So let us find out list of applications to learn more about gel filtration chromatography.

Some of the applications of Gel Filtration Chromatography are:

1. Gel filtration chromatography separates proteins by molecular size.
2. Smaller molecules enter gel pores and travel more slowly.
3. Larger molecules bypass pores and elute earlier in fractions.
4. The separation occurs without requiring protein–matrix binding interactions.
5. It preserves protein structure because no harsh chemicals are needed.
6. Gel filtration provides high resolution for macromolecular size differences.
7. The method enables estimation of unknown protein molecular weights.
8. It is widely used for analyzing enzyme oligomerization states.
9. Gel filtration allows clear distinction between monomers and aggregates.
10. The technique works under native conditions for sensitive biomolecules.
11. It supports both analytical characterization and preparative purification.