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Characteristics of High Performance Liquid Chromatography HPLC
Some characteristics of high performance liquid chromatography HPLC are:
- High-performance liquid chromatography forces mobile phases through packed columns which produces high separation efficiency.
- It applies controlled pressure delivery which stabilizes solvent transport through the column.
- It employs small stationary-phase particles which increase analyte–surface contact.
- It regulates flow velocity which reduces band dispersion during elution.
- It utilizes defined stationary-phase chemistry which governs analyte retention behavior.
- It controls mobile-phase composition which improves run-to-run reproducibility.
- It uses precision solvent pumping.
- It maintains controlled column temperature.
- It enables rapid column equilibration.
- It supports detector compatible separation.