Characteristics of High Performance Liquid Chromatography HPLC

Some characteristics of high performance liquid chromatography HPLC are: 

  1. High-performance liquid chromatography forces mobile phases through packed columns which produces high separation efficiency.
  2. It applies controlled pressure delivery which stabilizes solvent transport through the column.
  3. It employs small stationary-phase particles which increase analyte–surface contact.
  4. It regulates flow velocity which reduces band dispersion during elution.
  5. It utilizes defined stationary-phase chemistry which governs analyte retention behavior.
  6. It controls mobile-phase composition which improves run-to-run reproducibility.
  7. It uses precision solvent pumping.
  8. It maintains controlled column temperature.
  9. It enables rapid column equilibration.
  10. It supports detector compatible separation.