How Does Pore Size Affect Gel Filtration Chromatography?

Points on how pore size affect gel filtration chromatography are:

  • The porous gel matrix contains beads with a defined pore size range that determines which molecules are separated effectively. 
  • Only molecules within this pore size range can partially access the internal volume of the gel beads.
  • This pore-size control ensures that separation depends strictly on molecular size differences.

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