How Is Elution Volume Related to Size in Gel Filtration Chromatography?

Points on elution volume related to size in gel filtration chromatography:

• The elution volume in gel filtration chromatography is directly related to the fraction of pore volume accessible to a molecule. 
• Molecules that access more pore volume require a larger volume of mobile phase to pass through the column. 
• This relationship reflects size-dependent distribution within the porous gel matrix.

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