Importance of Degassing in HPLC

Some points on importance of degassing in HPLC are:

• Degassing in HPLC is important because it removes dissolved gases from the mobile phase and prevents bubble formation during pumping and detection.
• Degassing eliminates dissolved oxygen, nitrogen, and air from the mobile phase before it enters the pump system.
• It reduces gas release caused by pressure changes inside the pump and solvent lines.
• Degassing stabilizes solvent flow by preventing gas expansion during high-pressure operation.
• It maintains consistent mobile phase density by removing entrapped gases prior to chromatographic separation.
• Degassing prevents bubble formation that can disrupt piston movement in reciprocating HPLC pumps.
• It avoids signal noise and baseline disturbances caused by bubbles passing through the detector flow cell.
• Degassing protects check valves and seals from cavitation effects induced by gas pockets.
• It ensures continuous and uninterrupted solvent delivery by preventing vapor lock in the pumping system.