What Acts as the Stationary Phase in Paper Chromatography?

Points on stationary phase in paper chromatography:

  • In paper chromatography, a thin layer of water retained by the paper functions as the stationary phase during separation. 
  • Cellulose fibers hold this water through hydrogen bonding and capillary retention even when solvent flows through the paper. 
  • This retained aqueous layer provides the stationary environment required for chromatographic partitioning.

Also check out related content on what is paper chromatography for more information.