Why Are Locating Agents Important for Rf Value Measurement?

Points on imporance of locating agents for Rf value measurement are:

• The visibility produced by a locating agent allows accurate measurement of Rf values for separated analytes. 
• Clear visualization makes it possible to precisely mark the distance traveled by each compound on the chromatographic medium. 
• Accurate Rf determination depends on the reliable detection provided by the locating agent.

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