Why Is Gel Filtration Chromatography Considered a Mild Technique?

Points on why gel filtration chromatography considered a mild technique:

  • Gel filtration chromatography allows separation under mild operating conditions that preserve the native structure of biomolecules. 
  • Size-based separation through pores does not require harsh solvents or strong interactions. 
  • This enables intact passage of sensitive molecules through the gel matrix.

Find more information on definition of gel filtration chromatography to learn more about it.