How Do You Perform Column Chromatography Step-by-Step?

Points to perform column chromatography step by step:

• In column chromatography, the separation process begins by loading the sample mixture onto the top of the packed bed, followed by continuous elution with the liquid solvent, which gradually transports different molecules through the system at varying rates.
• As elution progresses, separated compounds emerge from the bottom in sequential order based on their retention times, allowing collection of purified fractions in separate containers for further analysis or use.
• Careful execution of this procedure ensures high-quality separations and maximum compound recovery.

Find out more details on what is column chromatography to learn more about the given topic.