Significance of chromatography in protein analysis

Points on significance of chromatography in protein analysis:

• In protein analysis, chromatography uses differences in size, charge, or binding properties to separate proteins.
• By exploiting the distinct physicochemical characteristics of each protein, chromatography directs their movement through the separation system, causing them to migrate at different rates and form discrete fractions.
• Through selective interaction between proteins and the chromatographic medium, this technique organizes complex protein mixtures into individually resolved components for downstream analysis.

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