Why is equilibrium important in partition chromatography?
Points on equilibrium in partition chromatography:
- Partition chromatography is based on equilibrium distribution, where each compound establishes a characteristic balance between the stationary liquid phase and the mobile phase.
- This balance is reached when the rate of transfer of a solute into the stationary phase equals the rate of transfer back into the mobile phase.
- Differences in these equilibrium states cause components to occupy distinct positions as separation proceeds.
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