Function of High Performance Liquid Chromatography HPLC

Some points on function of high performance liquid chromatography are:

• Clear spacing between neighboring chromatographic peaks confirms that HPLC separates multiple compounds without signal overlap.
• This spacing indicates sufficient resolution, meaning adjacent compounds are retained far enough apart along the chromatographic axis.
• When baseline separation is observed, each compound’s presence is confirmed independently rather than inferred from blended responses.

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