How Does Adsorption Chromatography Create Separation?

Adsorption chromatography creates separation when compounds compete for a limited number of active adsorption sites. The solid stationary phase provides a fixed surface with specific active centers where molecules can temporarily bind. When multiple compounds enter the column, they compete for these sites based on their polarity and surface affinity. Molecules that bind more strongly occupy the sites longer, slowing their movement through the column. Compounds with weaker attraction detach more quickly and travel faster with the mobile phase, resulting in clear separation as each substance moves at a different rate. You might also like importance of adsorption chromatography so check it out.