How Does Adsorption Chromatography Separate Similar Molecules?

Adsorption chromatography produces clear separation even when compounds differ only slightly in their polarity. In adsorption chromatography, the solid stationary phase responds to subtle differences in molecular structure and polarity. Small variations in functional groups change how strongly each compound binds to the surface. These minor differences affect retention time as some molecules remain adsorbed longer than others. As the mixture moves through the column, these gradual retention differences create distinct and visible separation bands. Also check out importance of adsorption chromatography for more information.