How does column chromatography transform separation into purification?

Some points on how column chromatography transform separation into purification:

  • During column chromatography, separated bands form at different positions in the column, and each collected fraction contains fewer impurities than the original mixture.
  • As elution progresses, individual components exit the column at different times, allowing fractions to be collected without overlap.
  • The identity of each fraction becomes clearer as separation progresses, rather than purity appearing all at once.

Find out more details on importance of column chromatography to learn more about it.