How Does a Liquid Stationary Phase Improve Separation in Partition Chromatography?

Some points on how liquid stationary phase improvs separation in partition chromatography:

  • The use of a liquid stationary phase in partition chromatography allows fine discrimination between structurally similar molecules.
  • Because solutes dissolve into the stationary liquid rather than adsorb onto a solid surface, small differences in solubility create distinct partition behavior.
  • This liquid–liquid distribution remains uniform throughout the column, enabling subtle structural differences to translate into reliable chromatographic separation.

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