How Does Low Polarity Contrast Still Produce Separation?

Some points on how low polarity constrast still produce separation:

• Partition chromatography produces clear separation when polarity contrast is too small for techniques relying on surface adsorption.
• Even minimal polarity differences change how compounds distribute between the stationary and mobile liquid phases.
• Through repeated equilibrium partitioning along the column, these small distribution differences accumulate into distinct chromatographic separation.

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