How Does Partition Chromatography Separate Similar Compounds?

Some points on how partition chromatography separate similar compounds:

• Partition chromatography separates compounds with nearly identical chemical structures by exploiting small differences in their distribution between two immiscible phases.
• Even when compounds share similar functional groups and molecular size, slight variations in solubility between the stationary and mobile phases produce distinct retention times.
• This distribution-controlled mechanism operates independently of surface adsorption, allowing subtle equilibrium differences to accumulate and result in clear chromatographic resolution.

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