How Does Partition Chromatography Separate Complex Mixtures?
Some points how partition chromatography separate complex mixtures:
- Partition chromatography separates complex mixtures by allowing multiple components to distribute independently between two immiscible liquid phases.
- Each compound establishes its own partition coefficient based on polarity and solvation preferences within the two liquids.
- As these independent equilibria repeat along the column, complex mixtures resolve into distinct chromatographic bands within a single analysis.
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