How Does Uneven Phase Distribution Drive Separation in Partition Chromatography?

Some points on how uneven phase distribution drive separation in partition chromatography:

• Partition chromatography separates compounds with small polarity differences by allowing each component to distribute unevenly between polar and nonpolar liquid phases.
• This unequal distribution changes how long each compound remains in the stationary phase versus the mobile phase during migration.
• As repeated partitioning occurs along the column, these time differences expand into clearly separated elution bands.

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