Importance of Ion Exchange Chromatography

Some points on importance of ion exchange chromatography:

  1. Ion-exchange chromatography is important because it allows selective purification and maintains high-resolution separation of charged molecules in complex mixtures.
  2. It isolates specific charged molecules even when many similar compounds exist in the same mixture.
  3. It separates charged molecules while leaving neutral substances behind in mixed samples.
  4. It purifies target charged molecules directly from crude sources such as cell extracts or reaction mixtures.
  5. It separates closely spaced charged components into clearly defined, distinct fractions
  6. It produces consistent and precise separation results across repeated runs of the same sample.
  7. It forms sharp separation boundaries that prevent overlap between neighboring charged species.