Significance of High Performance Liquid chromatography HPLC

Some points on significance of high performance liquid chromatography are:

• HPLC separates compounds with closely related chemical structures that would otherwise elute together in complex mixtures.
• This capability comes from adjustable stationary-phase interactions that respond to subtle differences in polarity or functional groups.
• Because of this selectivity, compounds that appear identical in bulk analysis can be resolved into separate chromatographic signals.

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