Why Does Partition Chromatography Work When Adsorption Methods Fail?

Some points on why partition chromatography work when adsorption methods fail:

• Partition chromatography distinguishes compounds that differ marginally in polarity when adsorption-based methods show poor resolution.
• Instead of relying on surface interactions, it separates compounds through repeated distribution between two immiscible liquid phases.
• This equilibrium-driven process amplifies small polarity differences over time, resulting in improved resolution for closely related compounds.

Also check it out related article on importance of partition chromatography for more information.