Why Does Separation Efficiency Remain Stable in Partition Chromatography?

Some points on why separation efficiency remain stable in partition chromatography:

  • Partition chromatography maintains separation efficiency even when mixtures contain compounds with overlapping chemical properties.
  • Efficiency is preserved because each compound repeatedly partitions between two immiscible liquid phases according to its own equilibrium behavior.
  • This continuous re-equilibration prevents loss of resolution, allowing stable and consistent separation throughout the chromatographic run.

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