Brief introduction about what is chromatography
what is Chromatography - Chromatography is one of the method which is used for the isolation or separation of different constituents of a solution mixture. There are many methods used for the separation of unknown sample from a mixture, Chromatography is one of them. It is used for the qualitative as well as in quantitative analysis. The component to be separated is passed through mobile phase and the stationary phase. Based on the properties of a given solution the constituent is separated and analyzed.
Principle and working of chromatography
Compound to be separated is passed into the chromatography column along with mobile phase, stationary phase is present in the column, Now based on the interaction of different molecules of compounds the constituent is separated. The molecules moving in the column will have different interaction with the stationary phase which leads to the separation of similar molecules. The molecules which have strong interaction with the stationary phase will separate slower and the constituent which have weaker interaction with the stationary phase will move faster. In this way different types of test molecules are separated from each other as they move over the support material. The constituent to be separated must have solubility properties with the mobile phase. Different substance have different Rf Values, Now Rf value will explain more about what is chromatography ?
Types of Stationary Phase
Chromatographic separation is carried out with different stationary phase support such as, volatile gas (Gas Chromatography), Immobilized Silica on glass plates (Thin Layer Chromatography), Paper (Paper Chromatography) and liquids which may incorporate hydrophilic and insoluble molecules (Liquid Chromatography).
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Different Types Chromatography
- Liquid Chromatography
- Gas Chromatography
- Paper Chromatography
- Super Critical Fluid Chromatography
- Ion Exchange Chromatography
- Affinity Chromatography
- Thin Layer Chromatography.
- High performance Liquid Chromatography
- High performance Thin Layer Chromatography
- Column Chromatography