Why does partition chromatography use two immiscible phases?
Points on why does partition chromatography use two immiscible phases:
- Partition chromatography is a chromatographic separation technique in which solutes distribute between two immiscible phases, one acting as the stationary liquid phase and the other as the mobile phase.
- The stationary liquid phase in partition chromatography does not chemically bind solutes but allows them to dissolve to different extents based on their affinity for that phase.
- The mobile phase continuously carries solutes past the stationary liquid layer, enabling repeated phase-to-phase distribution that produces separation.
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