Why does repeated partitioning matter in partition chromatography?

Points on repeated partitioning in partition chromatography:

  • The defining feature of partition chromatography is that components separate by repeatedly partitioning between a stationary liquid phase and a mobile phase during migration. 
  • Each solute undergoes multiple transfer events between the two phases as it moves through the system, reinforcing differences in distribution behavior. 
  • This cumulative partitioning effect causes components with different phase affinities to progressively separate over the course of movement.

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