How Adsorption Differences Enable Resolution in Normal Phase Chromatography?

Points on how adsorption differences enable resolution in normal phase chromatography:

• In normal phase chromatography, differences in adsorption strength allow polar analytes with similar structures to be retained and resolved separately.
• Small variations in functional group orientation and surface polarity cause measurable differences in how long closely related polar compounds remain on the stationary phase.
• This differential adsorption translates into distinct elution profiles, making it possible to resolve structurally similar polar analytes within the same run.

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