How Does Ion Exchange Chromatography Distinguish Similar Charged Species?

Some points on how ion exchange chromatography distinguish similar charged species:

• Ion exchange chromatography distinguishes closely related charged species that are difficult to purify using other methods.
• It differentiates molecules with subtle charge differences that cause similar behavior in alternative techniques.
• It resolves species with nearly identical chemical properties by exploiting small variations in charge interactions.
• It enables separation where conventional methods fail to discriminate between closely matched charged components.

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