How Polarity Causes Band Separation in Normal Phase Chromatography?

Points on how polarity causes band separation in normal phase chromatography:

• In normal phase chromatography, mixtures containing compounds of different polarity separate into distinct bands because polarity determines their interaction strength with the stationary phase.
• Each compound in the mixture establishes a different equilibrium with the stationary phase, causing spatial separation as the mixture moves through the column.
• These differences in interaction strength prevent co-migration, resulting in clearly defined bands rather than a single combined zone.

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