How Polarity Determines Elution Order in Normal Phase Chromatography?

Points on how polarity determines elution order in normal phase chromatography:

• In normal phase chromatography, compounds elute in order of increasing polarity, with nonpolar substances moving faster than polar ones through the column.
• Less polar compounds pass through the polar stationary phase with minimal interaction, allowing them to exit the column earlier than more polar species.
• Greater analyte polarity increases interaction with the stationary phase, delaying elution and producing a predictable polarity-based sequence.

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