How Solvent Strength Affects Retention in Normal Phase Chromatography?

Points on how solven strength affects retention in normal phase chromatography:

• In normal phase chromatography, tuning solvent strength alters the retention of polar analytes without affecting nonpolar components to the same extent.
• Stronger solvents compete more effectively with polar analytes for interaction sites, reducing retention while leaving weakly interacting nonpolar compounds largely unchanged.
• This selective response to solvent strength allows retention of polar analytes to be adjusted independently, improving control over chromatographic behavior.

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