Why Normal Phase Chromatography Retains Polar Analytes Better Than Reversed Phase?

Points on why normal phase is better than reversed phase chromatography:

  • In normal phase chromatography, analytes that lack sufficient retention in reversed-phase systems show stable and selective retention due to polarity-based interactions.
  • This contrast arises because polar analytes interact more favorably with polar stationary environments than with the nonpolar surfaces used in reversed-phase methods.
  • As a result, normal phase chromatography provides consistent retention for compounds that elute too quickly under reversed-phase conditions.

Also check it out importance of normal phase chromatography to learn more about it.