Why Polar Analytes Retain in Normal Phase Chromatography?
Points on why polar analytes retain in normal phase chromatography:
- In normal phase chromatography, the polar stationary phase interacts strongly with polar functional groups, causing selective and prolonged retention of polar analytes.
- These interactions arise from hydrogen bonding and dipole–dipole forces between the stationary phase surface and polar analytes, increasing their residence time on the column.
- As a result, polar compounds experience stronger adsorption than nonpolar molecules, which explains their delayed movement through the stationary phase.
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