Monday, 8 April 2013

ADSORPTION CHROMATOGRAPHY




ADSORPTION CHROMATOGRAPHY is one type of process which is used for the separation of components in a mixture by introducing it into chromatography system, Based on the relative differences in adsorption of components to the stationary phase present in the chromatography column the mixture is separated.

Adsorption Chromatography

Now over here the components or the molecules which are present in the mixture travels with different rates due to the differences in the affinity towards the stationary phase. Now there is attachment seen between the stationary phase and the molecules of components to be separated. 

Based on the polarity of the compounds they are separated. Suppose if the compound and the stationary phase both are polar then the polar compound are separated or elute out late while non polar compounds elute early. Because if both compound and the stationary phase are polar then there will be stronger adsorption and hence they will take more time to separate.
Adsorption Chromatography image 3


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