Sunday, 21 April 2013

Solid-Liquid Chromatography

About : Solid-Liquid Chromatography  Dt: 21/04/2013  Solid-Liquid Chromatography

Chromatography  may be with solid stationary phase or with liquid stationary phase is one of the widely used method to separate analyte mixture of compounds in which the separation is based on the differences in polarity of compounds. The separation of the analyte mixture takes place because of the intermolecular competition between the stationary phase and the mobile phase.

The Stationary phase used over here does not move as the name suggests while the moving phase is Mobile phase and the Moving analyte or compounds to be separated. The most common stationary phase used is made up of silica (Extremely fine particles of silica).

Two types of forces adsorbs other molecules on stationary phase hydrogen-bonding and dipole-dipole interactions. The more polar the analyte compound it will adsorb or adhere  to the solid stationary phase more strongly and will move slowly and will separate or elute out later. While non-polar compound will separate out quickly.The mobile phase consist of liquid solvents in which the analyte or compound is miscible.

There are two main types of liquid-solid chromatography Thin layer chromatography (TLC) and Column Chromatography