Advantages and Disadvantages of Paper Chromatography

Some advantages and disadvantages of paper chromatography are discussed below. So let us check it out its advantages and disadvantages to know more about paper chromatography.

Some advantages of Paper Chromatography are:

1. Paper Chromatography is simple, inexpensive and senstive method.
2. Minimal equipment required during paper chromatography.
3. Low cost materials are used during paper chromatography.
4. No specialized training needed.'
5. Small sample sizes.
6. Easy to scale up.
7. Paper chromatography gives quick results.
8. Efficient method of separation.
9. Versatile applications.
10. Widely available components. 

Some disadvantages of Paper Chromatography are:

1. Paper chromatography cannot handle large quantity of sample.
2. Paper chromatography has limited sample capacity.
3. It has long separaton time compared to other methods.
4. Low resolution for complex mixtures.
5. Difficult to maintain uniformity in paper chromatography.
6. Saturation of paper.
7. Large equipment needed for scalability.
8. It is expensive to upscale.

Advantages and Disadvantages of HPLC

 Some advantages and disadvantages of high performance liquid chromatography are discussed below. So let us check it its advantages and disadvantages to know more about HPLC.

Some advantages of HPLC chromatography:

1. HPLC has high resolution and speed of analysis.
2. High surface area.
3. It has high pressure gradient.
4. It has wide range of stationary phases.
5. Precise flow rate control.
6. Senstive detection methods.
7. Low sample method requirement.
8. Accurate peak identification using HPLC. 

Some disadvantages of HPLC chromatography

1. HPLC has high cost.
2. High quality components are needed.
3. The solvents and columns used in HPLC are expensive. 
4. Regular maintenance and calibration is needed which add extra cost.  
5. Sophisticated software is required for data analysis.
6. Research and development cost.