Wednesday, 12 December 2012

Steps Involved in HPTLC


High performance Thin layer chromatography is commonly known as (HPTLC). This method is used for estimation of both qualitative and quantitative of constituents mixture.

Some of the important steps involved in this separation are as follows:-

  1. Sample Preparation
  2. Selection of chromatographic layer.
  3. Plates
  4. Pre-washing
  5. Conditioning
  6. Pre-conditioning
  7. Mobile phase
  8. Chromatographic development
  9. Detection of spots
  10. Scanning and documentation.

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